bio[box] consists of a primary facility (DeGrace Hall room 103) and a microscope facility (Millis Hall room 315).  The following instruments are available for use by registered users with proper training.  Registration, training and scheduling for equipment use can be arranged through iLab .

Instrument Function Location
AATI Fragment Analyzer High-throughput capillary electrophoresis for accurate nucleic acid sizing, quantification, and QC DeGrace 103
Applied Biosystems Quantstudio 3 qPCR system Real-time PCR for relative quantification of target DNA and RNA sequences DeGrace 103
Bio-Rad C1000 thermal cycler Standard and deep-well PCR for DNA amplification DeGrace 103
Bio-Rad Chemidoc gel imager Fluorescent, chemiluminescent, and colorimetric imaging of gels and blots DeGrace 103
Bio-Rad QX200 ddPCR platform Droplet digital PCR for absolute quantification of target DNA and RNA sequences, including rare sequences and sequences in samples with PCR inhibitor carryover DeGrace 103
Labconco PCR workstation Positive-pressure workspace for low-contamination preparation of PCR samples, with HEPA filter and UV decontamination DeGrace 103
MilliQ Integral 10 water purification system Preparation of molecular biology-grade (DNase/RNase/pyrogen-free) water DeGrace 103
Molecular Devices Spectramax plate reader Absorbance, luminescence, and flourescence readings in 96-well format, with syringe injection and timecourse modes DeGrace 103
PCR workstation UV-equipped positive-pressure workstation for setting up PCR plates with low contamination. DeGrace 103
Qubit 3 fluorometer Highly quantitative dye-based measurement of DNA, RNA, and protein concentration in single samples DeGrace 103
Leica DM4B upright microscope with Leica DFC7000 T camera system Upright, manual light imaging at 4–40X magnification, with single and multichannel image acquisition Millis 15
Leica DMi8 inverted microscope with Leica DFC7000 T camera system  Automated, live cell imaging at 4–100X magnification, with multichannel image acquisition and image stitching Millis 315
Olympus BX60 upright microscope with Olympus DP72 camera system Upright, manual fluorescence imaging at 4–40X magnification, with single and multichannel image acquisition Millis 315